Stem-cell Based Therapies for Epidermolysis Bullosa
نویسندگان
چکیده
Direct reprogramming of somatic cells into induced pluripotent stem cells (iPSCs) provides an opportunityto develop novel personalized treatment options for numerous diseases and to advance current approachesfor cell-based drug discoveries and disease modeling. The ability to differentiate iPSCs into relevant celltypes is an important prerequisite for the successful development of iPSC-based treatment and modelingstrategies. Here, we describe a protocol for the efficient differentiation of human iPSCs into functionalkeratinocytes. The protocol employs treating iPSCs with retinoic acid and bone-morphogenetic protein-4to induce differentiation toward a keratinocyte lineage, which is then followed by the growth of differ-entiated iPSCs on collagen type Iand collagen type IV-coated dishes to enrich for iPSC-derivedkeratinocytes.
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